Reaction of the aminic form of aspartate transaminase with difluoro-oxaloacetate

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Dffluoro-oxaloacetate with Aspartate Transaminase

Difluoro-oxaloacetate interacts with the aldimine form of aspartate transaminase to give a complex, the dissociation constant ofwhich has been determined spectrophotometrically and by 19F n.m.r. (nuclear magnetic resonance). The 19F n.m.r. line-width-pH and chemical-shift-pH profiles of difluoro-oxaloacetate in the presence of the aldimine form of the enzyme both show inflexion points in the pH...

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ژورنال

عنوان ژورنال: Biochemical Journal

سال: 1977

ISSN: 0264-6021

DOI: 10.1042/bj1670193